의생명시스템과학과

KANGWON NATIONAL UNIVERSITY

Neurodevelopmental Biology Laboratory

Yun Kee, Ph.D.

• Email address : yunkee@kangwon.ac.kr
• Office: College of Biomedical Sicence, B-205
• Laboratories: College of Biomedical Science, B-201 & B-217
• Zebrafish facility : Center for Zebrafish Research

Field: Neurodevelopmental Biology & Embryology

Research Goals

• Kee lab research aims to
  1. reveal the functional roles of the genes, which are essential for tissue development during zebrafish embryogenesis, and understand the mechanisms of how these genes make connections in regulatory networks using Genetics & Genomics, Bioinformatics
  2. build the zebrafish disease models to mimic the pathological status of human diseases including neurodegenerative diseases and cancers and use them to understand the basis of disease pathology and screen the drug candidates

Research Projects

• Zebrafish Embryology & Developmental Biology using genome engineering tools including TALEN과 CRISPR/RGEN
• Gene knockdown technology
• Imaging: confocal microscopy and light-sheet microscopy (SPIM)
• Zebrafish disease models
• Development of cosmetic materials and toxicity test using zebrafish embryos

Lab Imaging of zebrafish embryos

present members

past members

Lab Photo Gallery

Published Articles

• Kim, M., Cho, H., Kwon, G., Kang, Y., Kwon, S.,Her, S., Kee, Y., Park, J.H.Y. (2015) Benzyl isothiocyanate suppresses high-fat diet-stimulated mammary tumor progression via the alteration of tumor microenvironments in obesity-resistant BALB/c mice. Mol Carcinogen, 54(1):72-82
• Kwon, S., Park, O.K., Nie, S., Kwak, J., Hwang, B.J., Bronner, M.E. and Kee, Y. (2014) Bioinformatic analysis of nematode migration-associated genes identifies novel vertebrate neural crest markers. PLOS One, 9(7), e103024
• Islam A.M.T., Kwak, J., Jung, Y.J., and Kee, Y. (2014) Animal models of Amyotrophic lateral sclerosis and Huntington's disease. Genes Genomics 36, 399-413
• Raslan, A.A. and Kee, Y. (2013) Tackling neurodegenerative disease: animal models of Alzheimer's disease and Parkinson's disease. Genes Genomics 35, 425-40
• Kee, Y. and Kim, N. (2013) Meeting report: Genetics and genome engineering. Genes Genomics 35, 411-3
• Kim, M.J., Park, S.K., Choi, V.N., Seo,S., Lee, S., Kee, Y. and Hwang, B. (2013) Enzymatic production and expression of shRNAmir30 from cDNAs. Genes Genomics 35, 395-403
• Kwak,J., Park,O.K., Jung, Y.J., Hwang, B., Kwon, S. and Kee, Y. (2013) Live image profiling of neural crest lineages in zebrafish transgenic lines. Mol Cells 35, 255-60
• Nie, S., Kee, Y. and Bronner-Fraser, M. (2011) Caldesmon regulates actin dynamics to influence cranial neural crest migration in Xenopus.Mol Biol Cell 22, 3355-65
• Nie, S., Kee, Y. and Bronner-Fraser, M. (2009) Myosin X is critical for the migratory ability of Xenopus cranial neural crest cells. Dev Biol 335, 132-42
• Demyanenko, A.V., Zhao, L., Kee, Y., Nie, S., Fraser, S.E. and Tyszka, J.M. (2009) A uniplanar three-axis gradient set for in vivo magnetic resonance microscopy. J Mag Res 200, 38-48
• Kee, Y., Hwang, B., Sternberg, P.W. and Bronner-Fraser, M. (2007) Evolutionary conservation of cell migration genes: from nematode neurons to vertebrate neural crest. Genes Dev 21, 391-6.
• Hoeling, B.M., Feldman, S.S., Strenge, D.T., Bernard, A., Hogan, E.R., Petersen, D.C., Fraser, S.E., Kee, Y., Tyszka, J.M., and Haskell, R.C. (2007) A Motion-sensitive 3D optical coherence microscope operating at 1300 nm for the visualization of early frog development. Proc of SPIE 6429, 64292T-1-9.
• Kee, Y. and Bronner-Fraser, M. (2005) To proliferate or to die: role of Id3 in cell cycle progression and survival of neural crest progenitors. Genes Dev 19, 744-55.
• Kee, Y. and Bronner-Fraser, M. (2001) The transcriptional regulator Id3 is expressed in cranial sensory placodes during early avian embryonic development. Mech Dev109, 337-340.
• Kee, Y. and Bronner-Fraser, M. (2001) Temporally and spatially restricted expression of the helix-loop-helix transcriptional regulator Id1 during avian embryogenesis. Mech Dev 109, 331-335.
• Kee, Y. and Bronner-Fraser, M. (2001) Id4 expression and its relationship to other Id genes during avian embryonic development. Mech Dev 109, 341-345.
• Hazuka C.D., Foletti D.L., Hsu S.C., Kee Y., Hopf F.W., and Scheller R.H. (1999) The sec6/8 complex is located at neurite outgrowth and axonal synapse-assembly domains. J. Neurosci19, 1324-1334.
• Kee Y.,Yoo J.S., Hazuka C.D., Peterson K.E., Hsu S.C., and Scheller R.H. (1997) Subunit structure of the mammalian exocyst complex. Proc. Natl. Acad. Sci. USA 94, 14438-14443.
• Hsu S.C., Ting A.E., Hazuka C.D., Davanger S., Kenny J.W., Kee Y., and Scheller R.H. (1997) The mammalian brain rsec6/8 complex. Neuron 17, 1209-1219.
• Kee Y. and Scheller R.H. (1996) Localization of synaptotagmin-binding domains on syntaxin. J. Neurosci 16, 1975-1981.
• Kee Y., Lin R.C., Hsu S.C., and Scheller R.H. (1995) Distinct domains of syntaxin are required for synaptic vesicle fusion complex formation and dissociation. Neuron 14, 991-998.
• Olney R.C., Smith R.L., Kee Y., and Wilson D.M. (1993) Production and hormonal regulation of insulin-like growth factor binding proteins in bovine chondrocytes. Endocrinology 133, 563-570.
• Nelson S.F., McCusker J.H., Sander M.A., Kee Y., Modrich P., and Brown P.O. (1993) Genomic mismatch scanning: a new approach to genetic linkage mapping. Nature Genetics 4, 11-18.
• Kee Y., Jung E.M., Seol J.H., Lee C.H., Ha D.B., and Chung C.H. (1990) Turnover of glycerol kinase during starvation in Escherichia coli. Mol Cells 1, 99-102.
• Kee Y., Park S.C., Ha D.B., and Chung C.H. (1989) Correlation between malignant phenotypes and changes in overall proteolytic capacity of human cervix and liver cancer. Korean J. Zool. 32, 48-54.
• Kee Y., Park S.C., Ha D.B., and Chung C.H. (1988) Relation of the activities of plasminogen activator and plasmin-like protease with malignant behavior of skin tumor of rats. Korean J. Zool. 31, 185-190.
• Kee Y., Lee Y.S., Goldberg A.L., and Chung C.H. (1988). Improved methods for purification and assay of glycerol kinase from Escherichia coli. J. Chromatography (Biomedical Applications) 428, 345-351.